产品概述：Purified recombinant protein of Mouse egl-9 family hypoxia-inducible factor 1 (Egln1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

蛋白长度：A DNA sequence from Mouse cDNA ORF Clone, [GM30590V], encoding Mouse full-length Egln1.

表达系统：HEK293T

种属：Mouse

标签类型：C-MYC/DDK

分子量：14.3 kDa

通用名：Egln1

总结：Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN1 is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. Target proteins are preferentially recognized via a LXXLAP motif.[UniProtKB/Swiss-Prot Function]

形式：Liquid

浓度：>50 ug/mL as determined by microplate BCA method

纯度：> 80% as determined by SDS-PAGE and Coomassie blue staining

储存溶液：25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

别名：AI503754; C1orf12; Hif-p4h-2; HIF-PH2; HPH-2; ORF13; Phd2; SM-20